The indirect enzyme-linked immunosorbent assay (ELISA) for detecting Herpes simplx virus (HSV) antibodies, IgG and IgM, was developed. HSV Type-1 strain Maclntyre, HSV Type-1 strain F, HSV Type-2 strain G and Herpes Type-2 strain MS were
propagated
on
human embryonic lung fibroblast (MRC-5) cells. Viral antigens were extracted from culture media and purified by sucrose density gradient ultracentrifugation.
Polystylene microwell plate was coated with purified HSV antigen (0.5ug/well) and blocked with BSA solution. This assay system was capable of detecting HSV-specific IgG and IgM antibodies in the sera in a short time(75 min). We compared this
assay
system with other commercially available EIA kits (Enzygnost;Behring, Germany). The overall sensitivity and specificity of IgG detection were 100% and 93.3%, respectively. The sensitivity and specificity of IgM detection were 97.9% and 99.3%,
respectively.
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